Soil Enzymatic Activity Measurements
Why measure soil enzymatic activity?
Hydrolytic extracellular soil enzymes are involved in key soil processes such as nutrient cycling, organic matter decomposition, as well as decomposition of soil pollutants. They are also very sensitive to soil anthropogenic management, which makes them a good candidate for modeling ecosystem responses to human activities.
What do we measure?
We simultaneously measure the activities of the five following hydrolytic enzymes:
β-Glucosidase
β-glucosidase (cellobiase, EC 3.2.1.21) is the most common glycosidase, important and widely used soil quality indicator. It is involved in the decomposition of cellulose and related polysaccharides.
β-N-Acetylglucosaminidase
β-N-Acetylglucosaminidase (E.C. 3.2.1.52; exochitinase) specifically hydrolyzes N-acetyl-glucosides e.g. release of glucosamine from the chitin.
Phosphomonoesterase
Phosphatase (E.C. 3.1.3.2) catalyses the hydrolysis of ester-phosphate bonds, which leads to the release of phosphate.
β-Xylosidase
β-Xylosidase (EC 3.2.1.37) involved in hydrolysis of hemicellulose (beta-D-xylans and xylobiose).
Leucine aminopeptidase
Leucine aminopeptidase (E.C. 3.4.11.1) catalyses the cleavage of amino acids of the amino terminus from protein or peptide substrates.
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Watch how to use our Soil Enzymatic Activity Reader
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Close-to-field Conditions:
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Minimal soil disturbance and no extraction steps.
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Error Reduction:
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Reduced handling errors for higher data accuracy and reliability.
Adaptability
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Included internal standards in each measurement to account for specific soil properties
Services
Digit soil offers several service options for your convinience.
Our system
Our in-laboratory measurement services assess the activities of five key soil enzymes simultaneously. We employ a novel, proprietary, patent-pending, contact-based approach that ensures minimal soil disturbance, close-to-field conditions, and fast results.
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Analyzer & Reaction Plates:
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Surface-based measurement of soil hydrolytic extracellular enzymes.
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Method validated with enzyme addition studies.
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User-Friendly Operation:
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Easy to operate with minimal sample preparation, guided by instructional videos.
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No wet lab required.
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Swift Results:
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Fast measurements yield enzymatic activity rates directly as output.
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Tailored Support:
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Close customer support: Direct communication with our scientific team.
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Flexibility to choose between on-site or off-site measurements.
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Innovation and Customization:
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Open for expansion on target enzymes to meet specific research needs.
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Open for integration with other sensors and measurement methods.